NEW TRENDS IN GC-FID TECHNIQUE FOR DETERMINATION OF NICOTINE IN THE ANTISMOKING PHARMACCEUTICAL FORMULATION
UDC 543.544; 547.596.2
Keywords:
liquid extraction, nicotine, аntismoking pharmaceutical formulations, chewing gum, transdermal patch, chromatographic analysisAbstract
A simple, rapid, accurate and eco-friendly analytical method was developed for the determination of nicotine in some pharmaceutical formulations. The method comprises a fast liquid extraction with organic solvent followed by direct capillary gas chromatography with flame ionization detector (GC-FID) separation and quantitation. Various experimental conditions, affecting on the efficiency of nicotine extraction (types of extracting solvents, volume, time of extraction, ultrasonic and thermal treatments) were investigated and optimized. Three various solvents (methanol, ethanol and chloroform) were tested and the best extraction from pharmaceutical formulations was attained with chloroform. The result for the recovery of nicotine from pharmaceutical formulations was in the range of 99 %. Under the optimal extraction condition of the quantitative recovery of nicotine from the patch by CHCI3 was achieved within 1 hour and chewing gum – 30 min. The ethanol extraction of nicotine from the antismoking pharmaceutical formulations is inapplicable, due to the partial oxidation of nico tine. This GC-FID method has been successfully applied to determine the nicotine content in both chewing and transdermal systems. The subsequent GC analysis does not consume any organic solvent as mobile phase while HPLC does. The limit of detection of the GC–FID method, established as three times of the noise of nicotine profile, was 0.25 mg/ml. The standard calibration curves were linear over the concentration range 0.0004–1000.0 mg/mL and with the relative standard deviations for chewing and transdermal systems were less than 0.4% and 2.0%, respectively.
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Copyright (c) 2016 Т. Губецька, cтуд., В. Левчик, канд. хім. наук, Н. Кобилінська, канд. хім. наук
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